Artigo
Purification, biochemical and functional characterization of miliin, a new thiol-dependent serine protease isolated from the latex of Euphorbia milii
Fecha
2008-07-01Registro en:
Protein and Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 15, n. 7, p. 724-730, 2008.
0929-8665
WOS:000259509600012
9162508978945887
6955258588672130
0000-0003-2460-1145
Autor
Universidade Estadual Paulista (Unesp)
Ctr Struct Mol Biol
Universidade de São Paulo (USP)
Faculdade de Medicina de São José do Rio Preto (FAMERP)
Universidade Federal de São Paulo (UNIFESP)
Resumen
Miliin, a new thiol-dependent serine protease purified from the latex of Euphorbia milii possesses a molecular weight of 79 kDa, an isoelectric point of 4.3 and is optimally active at 60 degrees C in the pH range of and 7.5-11.0. Activity tests indicate that milliin is a thiol-dependent serine protease.