Artigo
Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom
Fecha
2010-01-01Registro en:
Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 16, n. 4, p. 614-622, 2010.
1678-9199
S1678-91992010000400012
WOS:000284778000012
S1678-91992010000400012-en.pdf
8573195327542061
Autor
Universidade Federal do Ceará (UFC)
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
Universidade Federal da Paraíba (UFPB)
Resumen
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW (R) (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 mu g/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC(50) values of 1.94 mu g/mL and 5.49 mu g/mL. Furthermore, it repressed T. cruzi growth with an IC(50) of 1.14 mu g/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi.