Artigo
Selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation
Fecha
2012-09-01Registro en:
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 43, n. 3, p. 1062-1071, 2012.
1517-8382
10.1590/S1517-83822012000300032
S1517-83822012000300032
WOS:000311534700032
S1517-83822012000300032.pdf
9424175688206545
7091241742851920
0000-0002-4164-9362
Autor
Universidade Estadual Paulista (Unesp)
Resumen
Twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45ºC on medium containing corn straw and cardboard as carbon sources. These fungi were identified in the genera Aspergillus, Thermomyces, Myceliophthora, Thermomucor and Candida. The majority of the isolated strains produced xylanase and cellulases under solid state fermentation (SSF). The highest cellulase and xylanase productions were obtained by the cultivation of the strains identified as Aspergillus fumigatus M.7.1 and Myceliophthora thermophila M.7.7. The enzymes from these strains exhibited maximum activity at pH 5.0 and at 60 and 70ºC. The endo-glucanase from A. fumigatus was stable from 40ºC to 65ºC and both endo-glucanase and xylanase from M. thermophila were stable in this temperature range when in absence of substrate. The enzymes were stable from pH 4.0 to 9.0.