Artigo
New primers for the amplification and sequencing of nuclear loci in a taxonomically wide set of reptiles and amphibians
Fecha
2010-09-01Registro en:
Conservation Genetics Resources. Dordrecht: Springer, v. 2, p. 181-185, 2010.
1877-7252
10.1007/s12686-009-9126-4
WOS:000290413000044
0458077399058762
Autor
Univ Porto
Univ Vigo
Fed Univ Para
Universidade Estadual Paulista (Unesp)
Resumen
We report new primers for the amplification and sequencing of 11 nuclear markers in squamate reptiles and anuran amphibians (five in squamates, six in anurans). Ten out of the 11 loci are introns (three of which are linked) that were amplified using an exon-primed, intron-crossing (EPIC) PCR strategy, whereas an eleventh locus spans part of a protein-coding gene. Squamate and anuran primers were initially developed for Lacerta schreiberi (Squamata: Lacertidae) and Pelodytes spp. (Anura: Pelodytidae), respectively. Cross-species amplification of the squamate markers was evaluated in four genera representing two additional families, whereas for anurans three genera corresponding to three additional families were tested. Three out of the five loci were successfully sequenced in all squamate taxa tested. Cross-amplification of the six anuran markers had lower, but still significant, success. We predict these markers will be of great utility for both population genetics and phylogenetic studies.