Artigo
Seqüenciamento de peptídeos usando espectrometria de massas: um guia prático
Fecha
2008-01-01Registro en:
Química Nova. Sociedade Brasileira de Química, v. 31, n. 3, p. 669-675, 2008.
0100-4042
10.1590/S0100-40422008000300034
S0100-40422008000300034
WOS:000256956100034
S0100-40422008000300034.pdf
2901888624506535
Autor
Universidade de São Paulo (USP)
Fundo de Defesa da Citricultura (FUNDECITRUS)
Universidade Estadual Paulista (Unesp)
Resumen
This paper introduces the basics of peptide mass spectra interpretation applied to proteomics and is directed to chemists, biochemists and biologists. The manuscript presents a well detailed protocol aiming to serve as a first choice guide for understanding peptide sequencing. The tutorial was elaborated based on both a thorough bibliographic revision and the author's experience. In order to prove the applicability of the proposed guide, spectra obtained on different instruments have been successfully interpreted by applying the presented rational.