Artigo
Morphological and molecular characterization of Strongyloides ophidiae (Nematoda, Strongyloididae)
Fecha
2010-06-01Registro en:
Journal of Helminthology. Cambridge: Cambridge Univ Press, v. 84, n. 2, p. 138-+, 2010.
0022-149X
10.1017/S0022149X09990381
WOS:000278083500004
WOS000278083500004.pdf
3577149748456880
0000-0002-5929-1223
0000-0001-8735-6090
Autor
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Resumen
The aim of the present study is to report morphological data from parasitic female, rhabditoid and filarioid larvae, free-living female worms and eggs of Strongyloides ophidiae (Nematoda, Strongyloididae). In addition, a molecular DNA analysis was carried out using a pool of eight S. ophidiae parasitic females. Samples were obtained from the small intestine of Oxyrhopus guibei (Serpentes, Colubridae) collected in the municipality of Lencois Paulista, State of São Paulo, Brazil. DNA amplification by polymerase chain reaction (PCR) resulted in a 350 bp band for samples containing S. ophidiae and Strongyloides venezuelensis DNA. Strongyloides ophidiae nucleotide sequence analysis showed 98% similarity with Strongyloides procyonis and 97% with Strongyloides cebus, Strongyloides stercoralis, Strongyloides fuelleborni and Strongyloides sp. from snakes.