Artigo
Time interval from ovulation to extrusion in female bullfrog in different photoperiods
Fecha
2011-08-01Registro en:
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 40, n. 8, p. 1625-1628, 2011.
1516-3598
10.1590/S1516-35982011000800001
S1516-35982011000800001
WOS:000297416500001
S1516-35982011000800001.pdf
2772751144347110
0000-0003-0355-0553
Autor
Universidade Estadual Paulista (Unesp)
Cuesta Aquicultura
Resumen
It was analyzed in this work the influence of photoperiod on time interval from ovulation induction period to extrusion of ovocits in female bullfrogs (Lithobates catesbeianus). It was used 54 females reared from metamorphosis to 9 months of age under three photoperiods: dark time (DL 0:24), 16 hours of daylight (DL 16:8) and 12 hours of daylight (DL 12:12). Ovulation was induced by intramuscular application of two doses of LHRHa with 12 hours of interval between the injections. After 10, 25, 28, 31, 34 and 37 hours from the first hormone injection, 10-gram samples (3,000 eggs) were extracted from each female at each time interval and fertilized. Egg hatching rate was checked in each sample 72 hours after fertilization. Analysis of variance showed a significant effect of extrusion delay and the interaction between photoperiod and this delay. Extrusion should be carried out 33, 24 and 26 hours after the first hormone dosage in females reared in environments without light, with 12 hours of daylight and with 16 hours of daylight, respectively, to obtain the maximum fertilization rate.