Artigo
Immunological evaluation of the intestinal mucosa of broiler chicks treated with Lactobacillus Spp. and challenged with Salmonella Enteritidis
Fecha
2007-12-01Registro en:
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 9, n. 4, p. 259-262, 2007.
1516-635X
10.1590/S1516-635X2007000400009
S1516-635X2007000400009
WOS:000257537900009
S1516-635X2007000400009.pdf
8502462873517464
Autor
Universidade Estadual Paulista (Unesp)
Resumen
This study aimed at the antibody production by intestinal mucosa of broilers chicks were orally inoculated with Lactobacillus spp. at one and/or 21 days of age, and subsequently challenged with Salmonella enterica, subspecies enterica, serotype Enteritidis (S. Enteritidis). A total number of 288 drug-free broiler chicks was divided into 6 groups (groups A, B, C, D, E, and F), according to age at Lactobacillus spp. inoculation and SE challenge. The intestinal mucosa immune response was determined as the production of immunoglobulin A against S. Enteritidis, and evaluated by the Enzyme-Linked Immunosorbent Assay (ELISA) technique in intestinal washing fluid. Groups treated with Lactobacillus spp. presented higher IgA production only when the chicks were challenged with S. Enteritidis at 21 days of age. Nevertheless, the expected stimulus for intestinal mucosa antibody production induced by Lactobacillus spp was observed in only some of the treated groups, demonstrating that the protocol utilized in the present experiment resulted in few beneficial effects for chicks, particularly during the first days of life.