Artigo
Lack of effect of prior treatment with fluoride on genotoxicity of two chemical agents in vitro
Fecha
2007-01-01Registro en:
Caries Research. Basel: Karger, v. 41, n. 3, p. 239-243, 2007.
0008-6568
10.1159/000099326
WOS:000245758200014
5051118752980903
7528116925519142
Autor
Universidade Estadual Paulista (Unesp)
Universidade Federal de São Paulo (UNIFESP)
Resumen
The goal of this study was to investigate the ability of fluoride to modulate the genotoxic effects induced by the oxidative agent hydrogen peroxide (H2O2) and the alkylating agent methyl methanesulfonate (MMS) in vitro by the single-cell gel ( comet) assay. Chinese hamster ovary cells were exposed in culture for 1 h at 37 degrees C to sodium fluoride at 7-100 mu g/ml. NaF-treated and control cells were then incubated with 0-10 mu M MMS in phosphate-buffered saline (PBS) for 15 min at 37 degrees C, or 7-100 mu M H2O2 in distilled water for 5 min on ice. Negative control cells were treated with PBS for 1 h at 37 degrees C. Clear concentration-related effects were observed for the two genotoxins. Increase of DNA damage induced by either MMS or H2O2 was not significantly altered by pretreatment with NaF. The data indicate that NaF does not modulate alkylation-induced genotoxicity or oxidative DNA damage as measured by the single-cell gel ( comet) assay. Copyright (c) 2007 S. Karger AG, Basel