dc.contributor | Universidade de São Paulo (USP) | |
dc.contributor | Fac Med ABC | |
dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.contributor | Universidade Estadual Paulista (Unesp) | |
dc.date.accessioned | 2014-05-20T13:31:36Z | |
dc.date.accessioned | 2022-10-05T13:35:39Z | |
dc.date.available | 2014-05-20T13:31:36Z | |
dc.date.available | 2022-10-05T13:35:39Z | |
dc.date.created | 2014-05-20T13:31:36Z | |
dc.date.issued | 2012-10-30 | |
dc.identifier | Bmc Infectious Diseases. London: Biomed Central Ltd., v. 12, p. 8, 2012. | |
dc.identifier | 1471-2334 | |
dc.identifier | http://hdl.handle.net/11449/10767 | |
dc.identifier | 10.1186/1471-2334-12-278 | |
dc.identifier | WOS:000314094100001 | |
dc.identifier | WOS000314094100001.pdf | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3886857 | |
dc.description.abstract | Background: Cryptococcus neoformans causes meningitis and disseminated infection in healthy individuals, but more commonly in hosts with defective immune responses. Cell-mediated immunity is an important component of the immune response to a great variety of infections, including yeast infections. We aimed to evaluate a specific lymphocyte transformation assay to Cryptococcus neoformans in order to identify immunodeficiency associated to neurocryptococcosis (NCC) as primary cause of the mycosis.Methods: Healthy volunteers, poultry growers, and HIV-seronegative patients with neurocryptococcosis were tested for cellular immune response. Cryptococcal meningitis was diagnosed by India ink staining of cerebrospinal fluid and cryptococcal antigen test (Immunomycol-Inc, SP, Brazil). Isolated peripheral blood mononuclear cells were stimulated with C. neoformans antigen, C. albicans antigen, and pokeweed mitogen. The amount of H-3-thymidine incorporated was assessed, and the results were expressed as stimulation index (SI) and log SI, sensitivity, specificity, and cut-off value (receiver operating characteristics curve). We applied unpaired Student t tests to compare data and considered significant differences for p<0.05.Results: The lymphotoxin alpha showed a low capacity with all the stimuli for classifying patients as responders and non-responders. Lymphotoxin alpha stimulated by heated-killed antigen from patients with neurocryptococcosis was not affected by TCD4+ cell count, and the intensity of response did not correlate with the clinical evolution of neurocryptococcosis.Conclusion: Response to lymphocyte transformation assay should be analyzed based on a normal range and using more than one stimulator. The use of a cut-off value to classify patients with neurocryptococcosis is inadequate. Statistical analysis should be based on the log transformation of SI. A more purified antigen for evaluating specific response to C. neoformans is needed. | |
dc.language | eng | |
dc.publisher | Biomed Central Ltd. | |
dc.relation | BMC Infectious Diseases | |
dc.relation | 2.620 | |
dc.relation | 1,576 | |
dc.rights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Lymphocytes | |
dc.subject | Antigens | |
dc.subject | Biases | |
dc.subject | Statistical | |
dc.subject | Cryptococcus | |
dc.subject | Methods | |
dc.subject | Cryptococcus neoformans | |
dc.title | Lymphocyte transformation assay for C neoformans antigen is not reliable for detecting cellular impairment in patients with Neurocryptococcosis | |
dc.type | Artigo | |