Artigo
High-performance liquid chromatographic separation and identification of polyphenolic compounds from the infusion of Davilla elliptica St. Hill
Fecha
2008-01-01Registro en:
Phytochemical Analysis. Chichester: John Wiley & Sons Ltd, v. 19, n. 1, p. 17-24, 2008.
0958-0344
10.1002/pca.1008
WOS:000253340200002
3538253640602977
0000-0003-3032-2556
Autor
Universidade Estadual Paulista (Unesp)
Univ Salerno
Resumen
The isolation of polyphenolic compounds from an infusion of the Brazilian plant Davilla elliptica (Dilleniaceae), used as tea by virtue of its digestive properties, is described. An improved preparative HPLC method was used in order to isolate pure polyphenols from the complex mixture. Liquid-liquid extraction and solid-phase extraction were employed to minimise the interference of polymeric compounds and to provide an enriched fraction of the compounds of interest. The identification of the isolated compounds was performed using analytical HPLC as well as direct injection electrospray ionisation ion trap tandem mass spectrometry (ESI-IT-MS/MS). The high flavonoid content suggests that D. elliptica may be a promising source of compounds to produce natural phytomedicines. Copyright (C) 2007 John Wiley & Sons, Ltd.