Artigo
Purificação e caracterização de uma gentioexaose obtida de botriosferana por hidrólise ácida parcial
Fecha
2008-01-01Registro en:
Química Nova. Sociedade Brasileira de Química, v. 31, n. 5, p. 1015-1019, 2008.
0100-4042
10.1590/S0100-40422008000500013
S0100-40422008000500013
WOS:000259122200013
S0100-40422008000500013.pdf
Autor
Universidade Estadual Paulista (Unesp)
Universidade Estadual de Londrina (UEL)
Universidad de Castilla-La Mancha
Universidade de São Paulo (USP)
Resumen
A hexa-oligosaccharide was obtained by partial acid hydrolysis from botryosphaeran, an exopolysaccharide (EPS) β(1→3; 1→6)-D-glucan type, produced by the ascomyceteous fungus Botryosphaeria rhodina. The oligosaccharide was purified by gel filtration and charcoal-Celite column chromatography and the analysis was followed by HPAEC/PAD. The structure was determined by NMR spectroscopy and mass spectrometry, which showed that the oligosaccharide consists of six β-D-glucopiranosyl units O-6 substituted (gentiohexaose).