Artigo
Immobilization of uricase in layer-by-layer films used in amperometric biosensors for uric acid
Fecha
2007-11-01Registro en:
Journal of Solid State Electrochemistry. New York: Springer, v. 11, n. 11, p. 1489-1495, 2007.
1432-8488
10.1007/s10008-007-0314-4
WOS:000251676900005
Autor
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Resumen
The layer-by-layer technique was exploited to immobilize the enzyme uricase onto indium tin oxide substrates coated with a layer of Prussian Blue. Uricase layers were alternated with either poly(ethylene imine) or poly(diallyidimethylammoniumchloride), and the resulting films were used as amperometric biosensors for uric acid. Biosensors with optimum perfomance had a limit of detection of 0.15 mu A mu mol 1(-1) cm(-2) with a linear response between 0.1 and 0.6 mu M of uric acid, which is sufficient for use in clinical tests. Bioactivity was preserved for weeks, and there was negligible influence from interferents, as detection was carried out at 0.0 V vs saturated calomel electrode.