Dissertação
Estudo da composição química e atividade anti-inflamatória da fração diclorometânica e subfrações de partes aéreas de Echinodorus grandiflorus (Cham. & Schltd. Micheli)
Fecha
2018-07-30Autor
Marina Leão Souza Leite
Institución
Resumen
Echinodorus grandiflorus (Cham. & Schltdl.) Micheli, popularly known as chapéu de
couro in Brazil, is a plant species traditionally used to treat inflammatory diseases such
as arthritis and gout. The main goal of this study was to evaluate in vitro the effect of
E. grandiflorus dichloromethane fraction and subfractions on the production of
inflammatory mediators, as well as to investigate the chemical composition of the
active fraction. The crude extract of E. grandiflorus aerial parts was dissolved in water
(40 mg/mL) and partitioned with dichloromethane. This fraction was filtered over active
charcoal / celite (2:1) by eluting with methanol, acetone, dichloromethane and nhexane. The methanol and acetone sub-fractions are further fractionated by silica gel
column chromatography eluted with dichloromethane / ethyl acetate / methanol in
different proportions. The effect of the fractions were evaluated in vitro on LPSstimulated THP-1 cells. And the dexamethasone (0,1 µM/per well) was employed as
positive control. Among the assayed fractions, dichloromethane fraction reduced more
significantly the release of both mediators by LPS-stimulated THP-1 cells and showed
no cytotoxicity at all tested concentrations (10, 30, and 90 µg/mL). Fractionation of
methanolic sub-fraction by silica gel column chromatography afforded the sub-fraction
63-66, which tested at 10 µg/mL and 30 µg/mL elicited similar inhibition of TNF-α; and
IL-1β release. The chemical composition of sub-fraction 63-66 was investigated by
spectroscopy analyses (IR, GC-MS, UPLC-MS-MS and NMR) and allowed the
identification of palmitic acid (C16H32O2) as its main constituent. Our findings point out
Echinodorus grandiflorus as a source of bioactive compounds potentially useful to treat
inflammatory diseases.