Tese de Doutorado
Análise molecular de isolados do vírus da bronquite infecciosa das galinhas em Minas Gerais - Brasil
Fecha
2001-04-27Autor
Salette Lobao Torres Santiago
Institución
Resumen
In order to differentiate isolates of avian infectious bronchitis virus (IBV) in Minas Gerias state, Brasil, during period from 1972 to 1989, polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) and direct sequencing methods were used. Nine ereference IBV strains (eight serotypes) and 15 field isolates were studied. A sequence of about 1720 base pairs (bp) that contains the SI glycoprotein gene of IBV was amplified by PCR.