Tese de Doutorado
Controle da qualidade do ensaio de histamina em pescado
Fecha
2015-09-24Autor
Warlley Pinheiro Evangelista
Institución
Resumen
Histamine is a biogenic amine, that, at high levels, can cause adverse effects to human health. It has been associated to several cases of food intoxication in Brazil and throughout the world. It is also a parameter of quality and trade of tuna and related fish. Histamine analysis is required by the European Union (EU) for the importation of tuna. The official method of analysis (AOAC) is based on ion exchange chromatography and fluorimetric detection and the EU requires high pressure liquid chromatography (HPLC), recommending pre-column derivatization and ultraviolet detection. However, recent studies indicated that both methods are inadequate. The objective of this study was to implement the quality management system for histamine analysis in fish, validate the method for determination of histamine in fish and to provide data on histamine in tuna and freshwater fish. An extraction procedure was optimized and an ion-pair HPLC method was validated. It was considered efficient, precise and the limits of detection and quantification were fit for the purpose. The quality control techniques established by ABNT NBR ISO/IEC 17025:2005 were implemented and considered effective to warrant the quality of histamine analysis in tuna and other fishes. Canned samples from the market were also analyzed. The Quality Management System was implemented and the method was accredited by INMETRO. A quick Elisa method for the screening of histamine in fish was compared to the HPLC method proposed. Tuna samples from the states of Espírito Santo and Rio Grande do Sul and freshwater fish from Minas Gerais were analyzed providing excellent results for histamine. The method was used in the analysis of fresh tuna samples, canned and fresh water fish have demonstrating the effectiveness of method in various matrices.