A homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats

dc.creatorUZAL,F.A.
dc.creatorSMITH,P.
dc.creatorNIELSEN,K.
dc.creatorLIN,M.
dc.creatorKELLY,W.R.
dc.date1999-01-01
dc.date.accessioned2017-03-07T15:07:32Z
dc.date.available2017-03-07T15:07:32Z
dc.identifierhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0301-732X1999000100015
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/378349
dc.descriptionA fluorescence polarization assay (FPA) was developed to detect Clostridium perfringens type D epsilon antitoxin in serum of goats. Purified epsilon toxin was labelled with fluorescein isothiocyanate and used as a tracer in the test. Seven goat sera with different known concentrations of epsilon antitoxin (as measured by a mouse neutralization test-MNT) were used as positive controls. Sera from eleven colostrum-deprived kids were used as negative controls. The inter-assay variation of ten measurements of the seven positive sera varied between 1.56% and 4.85%. The correlation between the FPA values and concentration of epsilon antitoxin (as measured by the MNT) was 0.90. This test appears to be a good alternative to the MNT without most of the inconveniences of the later
dc.formattext/html
dc.languageen
dc.publisherFacultad de Ciencias Veterinarias, Universidad Austral de Chile
dc.sourceArchivos de medicina veterinaria v.31 n.1 1999
dc.subjectfluorescence polarization assay
dc.subjectgoats
dc.subjectClostridium perfringens
dc.subjectepsilon toxin antibodies
dc.titleA homogeneous fluorescence polarization assay for detection of Clostridium perfringenstype D epsilon antitoxin in serum of goats
dc.typeArtículos de revistas


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