Rojas, P (Rojas, P.)[ 1 ] ; Sandoval, C (Sandoval, C.)[ 3 ] ; Caligari, PDS (Caligari, P. D. S.)[ 1 ] [ 1 ] Univ Talca, Inst Biol Vegetal & Biotecnol, Talca, Chile [ 3 ] Univ Talca, Fac Ciencias Agr, Talca, ChileWild and cultivated Fragaria chiloensis ssp. chiloensis (Fcc) plants were collected at different locations in southern Chile in order to determine the current viral status of this native strawberry. The following aphidborne viruses (ABVs): Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry crinkle virus (SCV) and Strawberry vein banding virus (SVBV), were found in wild and cultivated Fcc plants, but severe symptoms were not associated with viral infection. Furthermore, partial gene sequences of these ABV isolates were determined and displayed a high degree of conservation with virus isolates reported previously. In addition, partial gene sequences of SCV and SVBV from southernmost South American populations of Fcc are described for the first time. High-throughput parallel sequencing (Illumina) of double-stranded RNA was used to provide viral profiles of Fcc from different locations. Although strong evidence of novel viruses affecting Fcc was not found, it was confirmed that ABVs are the most frequent viruses affecting this subspecies. The information provided will help in the development of high-quality molecular tools for virus detection and control in Fcc.; ; ;