Artículos de revistas
Development and preparation of novel carbon-11/fluorine-18 containing radiotracers for non-invasive PET-imaging of EphB4
Registro en:
JOURNAL OF LABELLED COMPOUNDS & RADIOPHARMACEUTICALS Volume: 56 Supplement: 1 Pages: S400-S400 Meeting Abstract: P313
0362-4803
Autor
Mamat, C.
Ebert, K.
Caballero, J.
Pietzsch, J.
Steinbach, J.
Institución
Resumen
Caballero, J (Caballero, Julio). Univ Talca, Ctr Bioinformat & Simulac Mol, Talca, Chile Meeting Abstract Objectives: [11C]Choline has become an extensively used tracer in part due to its wide accessibility via simple and
reliable synthetic protocols. The quality control for the tracer, however, seems still to be challenging. [11C]Choline
is commonly synthesized by passing [11C]methyl iodide through a SPE cartridge wetted with 2-
dimethylaminoethanol (DMEA), washing the cartridge with ethanol, water and eluting the product with saline.
Thus the aim for chromatography is to separate sodium chloride, residual DMEA and [11C]choline for the reliable
quantification DMEA, as it competes with [11C]choline in vivo. To achieve this, several chromatographic methods
have been reported, e.g. RP with ion-pair elution and IC in different combinations with RI, conductivity or
amperometric detection. The major difficulties listed were insufficient resolution and low detection sensitivity,
which at times required two chromatographic runs to accomplish the task [1-4]. We investigated the capability of
mixed-mode chromatography in resolving these issues.