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In vitro susceptibility of Microsporum spp and mammalian cells to Eugenia caryophyllus essential oil, eugenol and semisynthetic derivatives
Fecha
2020-05-19Registro en:
Autor
Leal Pinto, Sandra Milena.
Herrera Sandoval, Laura Viviana.
Vargas, Leonor Yamile.
Institución
Resumen
Background: Microsporum s
p
p are keratinophilic dermatophytes that mainly invade
the stratum corneum of the skin and hair causing clinical symptoms associated with
tinea. Its treatment has several limitations and the search for new active molecules is
necessary. Objetive: To evaluate the antifungal and cytotoxic potential of Eugenia
caryophyllus essential oil (EO), eugenol, isoeugenol and methylisoeugenol against
Microsporum canis, M. gypseum and Vero cells. Methods: The EO was extracted by
conventional heating assisted hydrodistillation
, the eugenol obtained commercially
and the derivatives through Williamson synthesis. Minimal Inhibitory Concentration
(MICs), minimum fungicidal concentration, inhibition of radial mycelial growth and
germination inhibition were used to evaluate the antifungal activity. In addition,
a
colorimetric test was conducte
d to evaluate cytotoxic activity. Results: MIC and MFC
values for all compounds were 62.5 to 500
μg/ml for both of the species of
Microsporum evaluated
.
Also, concentrations of 300
μg/ml of the compounds
inhibited 100% of M. canis mycelium. The inhibition of germination was observed
after 6 hours of treatment (11.86 ±3.46
-85.31 ±0 %). No cytotoxicity was observed in
Vero cells (CC50
> 105
μg/ml), whereas terbinafin
e showed CC50 31.00±0.61
μg/ml.
Conclusions: Our study indicates an interesting bioactivity of isoeugenol and
methylisoeugenol
against M. canis, M. gypseum and mammalian cells.