Complete Colombian Caribbean loggerhead turtle mitochondrial genome: tRNA structure analysis and revisited marine turtle phylogeny
Registro en:
instname:Universidad de Bogotá Jorge Tadeo Lozano
reponame:Repositorio Institucional de la Universidad de Bogotá Jorge Tadeo Lozano
Autor
Otálora, Katherin
Hernández-Fernández, Javier
Institución
Resumen
The loggerheadmarineturtle, Carettacaretta, isawidelydistributedand
endangeredspeciesthatisfacingcriticalpopulationdecline,especiallyin
Colombian Caribbeanrookeries.MitochondrialDNAsequencedataareof
greatimportanceforthedescription,monitoring,andphylogeneticanalysesof
migratoryturtlepopulations.Inthisstudy,thefirstfullmitochondrialgenomeofa
loggerheadturtlenestingintheColombianCaribbeanwassequencedandanalyzed.
This mitochondrialgenomeconsistsof16362bpwithanucleotidecompositionof
T:25.7%,C:27%,A:35%andG:12%.Sequenceannotationoftheassembled
molecule revealedanorganizationandnumberofcodingandfunctionalunits
as reportedforothervertebratemitogenomes.ThisColombianloggerheadturtle
(Cc-AO-C)showedanovelD-Loophaplotypeconsistingofthirteennewvariable
sites,sharing99.2%sequenceidentitywiththepreviouslyreportedCaribbean
loggerheadCC-A1D-Loophaplotype.All13protein-codinggenesintheCc-AO-C
mitogenomewerecomparedandalignedwiththosefromfourotherloggerhead
turtlesfromdifferentlocations(Florida,Greece,Peru,andHawaii).Elevenof
thesegenespresentedmoderategeneticdiversitylevels,andgenesCOIIandND5
showedthehighestdiversity,withaveragenumbersofpair-wisedifferencesof16.6
and 25,respectively.Inaddition,thefirstapproachrelatedtot-RNAs2Dand3D
structureanalysisinthismitogenomewasconducted,leadingtoobservedunique
featuresintwotRNAs(tRNATrp and tRNALeu). Themarineturtlephylogeny
wasrevisitedwiththenewlygenerateddata.Theentiremitogenomeprovided
phylogeneticallyinformativedata,aswellasindividualgenesND5,ND4,and16S.
In conclusion,thisstudyhighlightstheimportanceofcompletemitogenomedata
in revealinggeneflowprocessesinnaturalloggerheadturtlepopulations,aswellas
in understandingtheevolutionaryhistoryofmarineturtles.