Artículo de revista
Somatic embryogenesis in yam (dioscorea rotundata)
Fecha
2011Autor
Suárez Padrón, Isidro Elías
Torres Arizal, Luz Angela
Litz, Richard
Institución
Resumen
Embryogenic yam (Dioscorea rotundata) cultures were induced from petioles of leaves of in vitro grown plants on medium supplemented with different 2.4-D concentrations. Cultures were maintained either on semisolid or in liquid MS medium supplemented with 4.52 μM 2.4-D. The effect of sucrose concentration on somatic embryo development was also evaluated and the effects of different BAP concentrations on somatic embryo conversion were determined. Treatments were distributed using a complete randomized design. The highest rate of induction occurred with 4.52 μM 2.4-D. Sucrose at 131.46 mM significantly enhanced somatic embryo development. The conversion rate was not affected by BAP.