dc.creatorRetamal, Ignacio
dc.creatorHernández, Romina
dc.creatorVelarde, Victoria
dc.creatorOyarzún, Alejandro
dc.creatorMartínez, Constanza
dc.creatorGonzález Burgos, María Julieta
dc.creatorMartínez Winkler, Jorge
dc.creatorSmith, Patricio C.
dc.date.accessioned2020-04-29T14:25:34Z
dc.date.available2020-04-29T14:25:34Z
dc.date.created2020-04-29T14:25:34Z
dc.date.issued2020
dc.identifierOral Diseases Apr 2020
dc.identifier10.1111/odi.13325
dc.identifierhttps://repositorio.uchile.cl/handle/2250/174219
dc.description.abstractObjectives Myofibroblasts constitute a specific cell phenotype involved in connective tissue healing. Diabetes alters the wound healing response. However, it is not clear whether diabetes modifies the involvement of myofibroblasts in periodontal wounds. Materials and Methods Type I diabetes was induced in rats through streptozotocin injection, and periodontal wounds were performed. Wound healing was evaluated histologically at 2, 5, 7, and 15 days by measuring epithelial migration, neutrophil infiltration, and collagen and biofilm formation. Distribution of myofibroblasts was evaluated through immunofluorescence for alpha-smooth muscle actin. Data analyses were performed using the Shapiro-Wilk, ANOVA, or Kruskal-Wallis tests. Results Diabetic wounds were characterized by delayed epithelial closure, increased neutrophil infiltration, biofilm formation, and reduced collagen formation. Quantification of the myofibroblasts showed a significant reduction at 5 and 7 days in wounds of diabetic rats and an increase at 15 days when compared to wounds of non-diabetic rats. Conclusions Diabetic wound healing was associated with decreased epithelial and connective tissue healing, increased levels of inflammation, and biofilm formation. Myofibroblast differentiation was delayed in diabetic periodontal wounds at early time points. However, myofibroblasts persisted at later time points of healing. The present study suggests that diabetes alters the involvement of myofibroblasts during periodontal wound healing.
dc.publisherWiley
dc.sourceOral Diseases
dc.subjectCollagen
dc.subjectDiabetes
dc.subjectFibroblast
dc.subjectMyofibroblast
dc.subjectPeriodontal healing
dc.titleDiabetes alters the involvement of myofibroblasts during periodontal wound healing
dc.typeArtículo de revista


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