In vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas

dc.creatorBerland Olea, Marco
dc.creatorBaer, A.
dc.creatorRuíz, J.
dc.creatorParraguez, Víctor
dc.creatorMorales, P.
dc.creatorAdams, G.P.
dc.creatorRatto Fuster, Marcelo
dc.date2011
dc.date2012-02-07T23:40:02Z
dc.date2012-02-07T23:40:02Z
dc.date2012-02-07
dc.date.accessioned2021-06-14T22:07:05Z
dc.date.available2021-06-14T22:07:05Z
dc.identifierTheriogenology, Vol. 75, N°8, 1482-1488, 2011
dc.identifierhttps://hdl.handle.net/10925/589
dc.identifier10.1016/j.theriogenology.2010.11.047
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3300911
dc.descriptionThe objective was to evaluate the developmental competence of cumulus-oocyte complexes (COC) collected by follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly to two groups (n = 16 per group) and treated, at the time of ovarian follicular wave emergence, with either: 1) 25 mg of FSH im, twice daily for 4 d; or 2) 1000 IU of eCG as a single i.m. dose. The start of gonadotropin treatment was considered Day 0. Both groups were given 5 mg of Armour Standard LH im on Day 6, and COC were collected by follicle aspiration on Day 7. Expanded COC collected from FSH- (n = 157) and eCG-treated llamas (n = 151) were fertilized in vitro using epididymal sperm, and presumptive zygotes were in vitro cultured in SOF medium for 8 d. The FSH and eCG treatment groups did not differ with respect to: the number of follicles 7 mm (16.0; 2.7 vs 14.0; 1.9, respectively; P = 0.5); the number of COC collected (11.5; 1.9 vs 9.7; 1.2; P = 0.4); the number of expanded COC (9.8; 1.4 vs 9.4; 1.2; P = 0.8); or the percentage of presumptive zygotes which developed into 2 to 8 cell stage embryos (65.3 vs 63.1), morulas (46.2 vs 42.5), or blastocysts (23.1 vs 20.5; P > 0.05). In conclusion, FSH and eCG treatments were equally effective for recovery of a high number of expanded COC which were used directly for in vitro fertilization. Furthermore, rate of embryo development was not significantly affected by the gonadotropin treatment used. © 2011 Elsevier Inc.
dc.formatPDF
dc.formatapplication/msword
dc.languageen
dc.sourceTheriogenology
dc.subjectFertilización in vitro
dc.subjectSuperestimulación
dc.subjectGonadotropinas
dc.titleIn vitro fertilization and development of cumulus oocytes complexes collected by ultrasound-guided follicle aspiration in superstimulated llamas
dc.typeArtículo de Revista


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