Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch

dc.creatorMárquez Rodríguez, Lorenzo
dc.creatorHernández Arias, Adrián
dc.creatorCarvalho, Edison S. M.
dc.creatorMorales, Gabriel A.
dc.creatorDantagnan Dantagnan, Patricio
dc.creatorDiaz, Manuel
dc.creatorMoyano, Francisco J.
dc.date2019
dc.date2021-04-30T17:05:06Z
dc.date2021-04-30T17:05:06Z
dc.date.accessioned2021-06-14T22:06:14Z
dc.date.available2021-06-14T22:06:14Z
dc.identifierLATIN AMERICAN JOURNAL OF AQUATIC RESEARCH,Vol.47,860-864,2019
dc.identifierhttp://repositoriodigital.uct.cl/handle/10925/3968
dc.identifier10.3856/vol47-issue5-fulltext-16
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/3300575
dc.descriptionThe activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.
dc.languageen
dc.publisherUNIV CATOLICA DE VALPARAISO
dc.sourceLATIN AMERICAN JOURNAL OF AQUATIC RESEARCH
dc.subjectOncorhynchus kisutch
dc.subjectCoho salmon
dc.subjectgastric proteases
dc.subjectenzymatic activity
dc.subjectpH
dc.subjectionic strength
dc.titleEffects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
dc.typeArticle


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