The Austral Pygmy Owl (Glaucidium nana) is usually recorded as actively calling and foraging during daylight. We studied the diurnal activity of the Austral Pygmy Owl over I year in Cerro Nielol Natural Monument in southern Chile totaling 339 hrs of observation. The most intense activity was recorded at mid-morning between 0900 to 1200 hrs when conspicuous perching and foraging attempts were observed. Vocalizations showed a pattern associated with the reproductive season. The contact pair call was most used throughout the year (54%), slightly more than the territorial call (46%). Received 20 July 2011. Accepted 14 April 2012.