| dc.creator | Márquez Rodríguez, Lorenzo | |
| dc.creator | Fuentes, Juan | |
| dc.date | 2014 | |
| dc.date | 2021-04-30T16:32:58Z | |
| dc.date | 2021-04-30T16:32:58Z | |
| dc.date.accessioned | 2021-06-14T22:02:40Z | |
| dc.date.available | 2021-06-14T22:02:40Z | |
| dc.identifier | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,Vol.167,52-58,2014 | |
| dc.identifier | http://repositoriodigital.uct.cl/handle/10925/2963 | |
| dc.identifier | 10.1016/j.cbpa.2013.10.016 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/3299278 | |
| dc.description | The gastric acid secretion of juvenile Sparus aurata was characterized in Ussing chambers; secretion rates were determined by a pH-stat method at pH 5.50 and bioelectrical parameters were measured in current-clamped tissues. The basal secretion equaled to 535 +/- 87 nmol.cm(-2).h(-1). Serosal carbachol 100 mu M produced an increase (Delta J(H+)) of 725 +/- 133 nmol.cm(-2).h(-1) from basal secretion, this effect being inhibited by mucosal omeprazole 100 mu M. Basal secretion was also sensitive to the combination of serosal forskolin (FK) 10 mu M + serosal isobutylmethylxanthine (IBMX) 100 mu M (Delta J(H+) = 793 +/- 239 nmol.cm(-2).h(-1)); this effect was insensitive to mucosa( omeprazole 100 mM but inhibited by mucosal bafilomycin A1 100 nM. The effect of carbachol proceeded within a few minutes (<10 min), whereas the effect of FK + IBMX was gradual, taking 40 min to reach the maximum. The addition of mucosal gadolinium (Gd3+) 100 mu M, a potent calcium-sensing receptor (CaR) agonist, stimulated the basal secretion (Delta J(H+) = 340 +/- 81 nmol.cm(-2).h(-1)). The present results indicate that the add secretion mechanism in the sea bream stomach is regulated by muscarinic and CaR-like receptors, cAMP is implicated in the signal transduction, and at least two proton pumps, a HK-ATPase and a V-ATPase contribute to acid secretion. (C) 2013 Elsevier Inc. All rights reserved. | |
| dc.language | en | |
| dc.publisher | ELSEVIER SCIENCE INC | |
| dc.source | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | |
| dc.subject | Calcium-sensing receptor agonists | |
| dc.subject | cAMP promoters | |
| dc.subject | Gastric acid secretion | |
| dc.subject | HK-ATPase | |
| dc.subject | Muscarinic agonists | |
| dc.subject | Sparus aurata | |
| dc.subject | V-ATPase | |
| dc.title | In vitro characterization of acid secretion in the gilthead sea bream (Sparus aurata) stomach | |
| dc.type | Article | |
