Artículo de revista
ZnO-based nanofungicides : Synthesis, characterization and their effect on the coffee fungi Mycena citricolor and Colletotrichum sp.
Fecha
2019-05Registro en:
10.3171/2018.1.JNS171972
0022-3085
1933-0693
Autor
Arciniegas Grijalba, P.A.
Patiño Portela, M.C.
Mosquera Sánchez, L.P.
Guerra Sierra, Beatriz Elena
Muñoz Flórez, Jaime Eduardo
Erazo Castillo, L.A.
Rodríguez Páez, J.E.
Institución
Resumen
mical
route and a ZnO-based nanobiohybrid obtained by green synthesis in an extract of garlic (Allium sativum).
To find out the characteristics of the materials synthesized, X-ray diffraction (XRD), IR spectroscopy and absorption in UV–Vis were used, as well as both scanning (SEM) and transmission (TEM) electron microscopy. The results showed that the samples obtained were of nanometric size (< 100 nm), with a predominance of the
wurtzite crystal phase of ZnO and little crystallization of the nanobiohybrids. Their antifungal capacity on two
pathogenic fungi of coffee, Mycena citricolor (Berk and Curt) and Colletotrichum sp. was also evaluated. Both
nanomaterials showed an efficient antifungal capacity, particularly the nanobiohybrids, with ~97% inhibition in
growth of M. citricolor, and ~93% for Colletotrichum sp. The microstructural study with high resolution optical
(HROM) and ultra-structural microscopy (using TEM) carried out on the fungi treated with the synthesized
nanomaterials showed a strong nanofungicidal effect on the vegetative and reproductive structures and fungal
cell wall, respectively. The inhibition of the growth of the fungi and micro and ultra-structural affectations were
explained considering that the size of the nanomaterials allows them to pass easily through the cell membranes.
This indicates that they can be absorbed easily by the fungi tested here, causing cellular dysfunction.
Nanofungicide effects are also attributable to the unique properties of nanomaterials, such as the high surface-tobulk ratio of atoms and their surface physicochemical characteristics that could directly or indirectly produce
reactive oxygen species (ROS), which affect the proteins of the cell wall.