dc.contributorUniversidade Estadual de Maringá; Universidade Estadual de Maringá; Universidade Estadual de Maringá; Universidade Estadual de Maringá.
dc.creatorCOSTA, A. T.
dc.creatorNUNES, W. M. de C.
dc.creatorZANUTTO, C. A.
dc.creatorMÜLLER, G. W.
dc.date2014-08-02T07:00:22Z
dc.date2014-08-02T07:00:22Z
dc.date2010
dc.date2010-10-15
dc.date.accessioned2017-03-06T21:27:54Z
dc.date.available2017-03-06T21:27:54Z
dc.identifier48300
dc.identifierhttp://www.alice.cnptia.embrapa.br/handle/doc/864301
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/313115
dc.descriptionAbstract ? The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products electrophoretic profiles were analyzed using the Jaccard coefficient and the UPGMA method. The majority of the clones had weak to moderate stem pitting symptoms and its CTV isolates showed alterations in the SSCP profiles. However, the stability of the protective complex has been maintained, except for isolates from two analised clones. Low genetic variability was observed within the isolates during the studied years.
dc.description2010
dc.languageen
dc.publisherPesquisa Agropecuaria brasileira., Brasília, v.45, n.7, p.693-700, jul. 2010
dc.relationÁrea de Informação da Sede - Artigo em periódico indexado (ALICE)
dc.subjectCross-protection
dc.subjectPreimmunization
dc.subjectSingle strand conformational polymorphism
dc.subjectStem pitting symptoms
dc.subjectCitrus sinensis
dc.titleStability of Citrus tristeza virus protective isolates in field conditions.
dc.typeArtículos de revistas


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