Articulo
Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification
Autor
Querido, Jailson F. B.
Agirre, Jon
Martí, Gerardo Aníbal
Guérin, Diego M. A.
Silva, Marcelo Sousa
Institución
Resumen
Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus. Centro de Estudios Parasitológicos y de Vectores