Evaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus

dc.creatorEcheverría, María Gabriela
dc.creatorNosetto, Edgardo Omar
dc.creatorEtcheverrigaray, María Elisa
dc.date2000
dc.date2019-10-17T17:14:10Z
dc.identifierhttp://sedici.unlp.edu.ar/handle/10915/83521
dc.identifierissn:1040-6387
dc.descriptionA blocking enzyme-linked immunosorbent assay (ELISA) using a urease conjugate (U-B-ELISA) was evaluated for screening sera for antibodies to pseudorabies virus under field conditions, A total of 764 serum samples were analyzed by U-B-ELISA. Of these, 264 were evaluated by both virus neutralization and U-B-ELISA, and the results were compared. U-B-ELISA showed 98.5% and 98.9% sensitivity and specificity, respectively. This test combines the sensitivity and specificity of the blocking ELISA format while allowing visual assessment of results.
dc.descriptionFacultad de Ciencias Veterinarias
dc.formatapplication/pdf
dc.format266-268
dc.languageen
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rightsCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.subjectCiencias Veterinarias
dc.subjectpseudorabies virus
dc.subjectELISA
dc.subjectenzyme-linked immunosorbent assay
dc.titleEvaluation of a blocking ELISA using a urease conjugate for the detection of antibodies to pseudorabies virus
dc.typeArticulo
dc.typeArticulo


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