Articulo
Stimulation of adhesiveness, infectivity, and competitiveness for nodulation of Bradyrhizobium japonicum by its pretreatment with soybean seed lectin
Autor
Lodeiro, Aníbal Roberto
López García, Silvina Laura
Vázquez, Tirso Emiliano E.
Favelukes, Gabriel
Institución
Resumen
Soybean seed lectin stimulates adsorption of <i>Bradyrhizobium japonicum</i> to its host roots. Pretreatment of the rhizobia with soybean seed lectin for at least 6-12 h previous to their interaction with the plants was required to detect the stimulatory effect. This activity could be observed with as few as 1000 soybean seed lectin molecules per bacterium, and required specific carbohydrate binding. Infectivity and competitiveness for nodulation were also stimulated by preincubation of the rhizobia either with soybean seed meal extract or soybean seed lectin, the extract being more effective in enhancing competitiveness. Instituto de Biotecnologia y Biologia Molecular Facultad de Ciencias Exactas