Artículos de revistas
Improvement in carbohydrate and phlorotannin extraction from Macrocystis pyrifera using carbohydrate active enzyme from marine Alternaria sp. as pretreatment
Fecha
2017Registro en:
Journal of Applied Phycology, (2017) 29:2039–2048
15735176
09218971
10.1007/s10811-017-1141-3
Autor
Leyton, A.
Pezoa Conte, R.
Maki Arvela, P.
Mikkola, J. P.
Lienqueo, M. E.
Institución
Resumen
The commercial importance of brown seaweed hasbeen increasing over the past decade, especially due to industriesinterestedintheextractionofphycocolloidsand,morerecently,ofpolyphenol compounds such as phlorotannins. The objective ofthis work was to optimize the extraction conditions of carbohy-drates and phlorotannins fromMacrocystis pyrifera,evaluatedenzymatic pretreatment and different parameters of extractionusing design of experiment. The optimal conditions upon extrac-tion of the carbohydrates and phlorotannins were determined bymeans of a pretreatment protocol taking advantage on a carbohy-drate active enzyme, followed by an alkaline hydrolysis with0.5 N NaOH at 100 °C, 180 min, and S/L ratio of 1/20. In orderto extract the carbohydrates, the best conditions found for thepretreatment procedure were 37 °C, pH 7.0 for 24 h, and a S/Lratioof1/10,givinganextractionyield(EY)of89.67±12.3wt.%.In turn, for the extraction of phlorotannins, the best conditionsidentified in terms of the pretreatment were 25 °C, pH 7.0 for36 h, and a S/L ratio of 1/20, thus giving a yield (EY) of2.14 ± 0.25 wt.%. Statistical analysis of both processes revealeda maximum EYof 91.24 wt.% for carbohydrates and 3.31 wt.%EY for phlorotannins