Artículos de revistas
Diurnal changes in active carbon and nitrogen pathways along the temperature gradient in porcelana hot spring microbial mat
Fecha
2018Registro en:
Frontiers in Microbiology, Volumen 9, Issue OCT, 2018,
1664302X
10.3389/fmicb.2018.02353
Autor
Alcamán-Arias, María E.
Pedrós-Alió, Carlos
Tamames, Javier
Fernández, Camila
Pérez-Pantoja, Danilo
Vásquez, Mónica
Díez, Beatriz
Institución
Resumen
© 2007 - 2018 Frontiers Media S.A. All Rights Reserved. Composition, carbon and nitrogen uptake, and gene transcription of microbial mat communities in Porcelana neutral hot spring (Northern Chilean Patagonia) were analyzed using metagenomics, metatranscriptomics and isotopically labeled carbon (H13CO3) and nitrogen (15NH4Cl and K15NO3) assimilation rates. The microbial mat community included 31 phyla, of which only Cyanobacteria and Chloroflexi were dominant. At 58°C both phyla co-occurred, with similar contributions in relative abundances in metagenomes and total transcriptional activity. At 66°C, filamentous anoxygenic phototrophic Chloroflexi were >90% responsible for the total transcriptional activity recovered, while Cyanobacteria contributed most metagenomics and metatranscriptomics reads at 48°C. According to such reads, phototrophy was carried out both through oxygenic photosynthesis by Cyanobacteria (mostly Mastigocladus) and anoxygenic phototrophy due mainly to Chloroflexi.