Artículos de revistas
Intestinal inflammation in Chilean infants fed with bovine formula vs. Breast milk and its association with their gut microbiota
Fecha
2018Registro en:
Frontiers in Cellular and Infection Microbiology, Volumen 8, Issue JUN, 2018,
22352988
10.3389/fcimb.2018.00190
Autor
Ossa, Juan C.
Yáñez, Dominique
Valenzuela, Romina
Villarroel Gallardo, Pablo Antonio
Lucero, Yalda
Farfán, Mauricio J.
Institución
Resumen
© 2018 Ossa, Yáñez, Valenzuela, Gallardo, Lucero and Farfán. Introduction: Compared to bovine formula (BF), breast milk (BM) has unique properties. In the newborn intestine, there is a homeostatic balance between the counterparts of the immune system, which allows a physiological inflammation, modulated by the gut microbiota. Many studies have attempted to understand the effect of BF vs. BM, and the changes in the gut microbiota, but few also focus on intestinal inflammation. Methods: We conducted a cohort study of newborn infants during their first 3 months. In stool samples taken at 1 and 3 months (timepoints T1 and T3), we quantified calprotectin, IL-8 and a1-antitrypsin by ELISA and we evaluated the expression of IL8 and IL1β genes by RT-qPCR. To determine the microbiota composition, the 16S rRNA gene was amplified and sequenced using 454 pyrosequencing. Sequences were clustered into operational taxonomic units (OTUs). Results: In total 15 BM and 10 BF infants were enrolled. In the