Modeling and estimation of production rate for the production phase of non-growth-associated high cell density processes

dc.creatorJamilis Ricaldoni, Martín Ignacio
dc.creatorGarelli, Fabricio
dc.creatorMozumder, Salatul Islam
dc.creatorCastañeda, María Teresita
dc.creatorDe Battista, Hernán
dc.date2015-07-07
dc.identifierhttps://digital.cic.gba.gob.ar/handle/11746/7751
dc.identifierDocumento completo
dc.descriptionThis paper addresses the estimation of the specific production rate of intracellular products and the modeling of the bioreactor volume dynamics in high cell density fed-batch reactors. In particular, a new model for the bioreactor volume is proposed, suitable to be used in high cell density cultures where large amounts of intracellular products are stored. Based on the proposed volume model, two forms of a high-order sliding mode observer are proposed. Each form corresponds to the cases with residual biomass concentration or volume measurement, respectively. The observers achieve finite time convergence and robustness to process uncertainties as the kinetic model is not required. Stability proofs for the proposed observer are given. The observer algorithm is assessed numerically and experimentally.
dc.formatapplication/pdf
dc.format12 p.
dc.languageInglés
dc.relationDOI:10.1007/s00449-015-1430-7
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subjectObserver
dc.subjectSliding mode
dc.subjectHigh cell density
dc.subjectEstimation
dc.subjectVolume model
dc.titleModeling and estimation of production rate for the production phase of non-growth-associated high cell density processes


Este ítem pertenece a la siguiente institución