dc.contributor | Souza, Azair Liane Matos do Canto de | |
dc.contributor | http://lattes.cnpq.br/2352004564367849 | |
dc.contributor | http://lattes.cnpq.br/8438045901614166 | |
dc.creator | Souza, Vanessa Nunes de | |
dc.date.accessioned | 2012-05-31 | |
dc.date.accessioned | 2016-06-02T19:22:07Z | |
dc.date.available | 2012-05-31 | |
dc.date.available | 2016-06-02T19:22:07Z | |
dc.date.created | 2012-05-31 | |
dc.date.created | 2016-06-02T19:22:07Z | |
dc.date.issued | 2011-12-15 | |
dc.identifier | SOUZA, Vanessa Nunes de. Blockade of 5-HT2 receptors in the periaqueductal grey matter (PAG) abolishes the anxiolytic-like effect of 5-HT1A receptor antagonism in the median raphé nucleus in mice. 2011.. 2011. 73 f. Tese (Doutorado em Ciências Biológicas) - Universidade Federal de São Carlos, São Carlos, 2011. | |
dc.identifier | https://repositorio.ufscar.br/handle/ufscar/1236 | |
dc.description.abstract | Several lines of evidence support the involvement of serotonergic (5-HT) neurons of the median raphe nucleus (MRN) in anxiety-like behaviour. In this context, it is known that blockade of 5- HT1A somatodendritic autoreceptors in the midbrain raphe nuclei increases the firing rate of these neurons, disinhibiting 5-HT release in postsynaptic target areas such as amygdala, hippocampus and periaqueductal grey matter (PAG). However, while activation of 5-HT1A or 5- HT2 receptors in forebrain targets such as the amygdala or hippocampus enhances anxiety-like behaviours in rodents, stimulation of both receptor subtypes in the midbrain PAG markedly reduces anxiety-like behaviour. In view of these findings, the present study investigated whether the anti-anxiety effects induced by pharmacological disinhibition of 5-HT neurons in the MRN are attenuated by the blockade of 5-HT2 receptors within the PAG. Mice received combined intra-PAG injection with ketanserin (10 nmol/0.1 μl), a 5-HT2 receptor antagonist, followed by intra-MRN injection of WAY-100635 (5.6 nmol/0.l μl), a highly selective 5-HT1A receptor antagonist. They were then individually exposed to the elevated plus-maze (EPM), with the videotaped behavioural sessions subsequently scored for both conventional and ethological measures. The results confirmed that intra-MRN infusion of WAY100635 (5.6 nmol/0.l μl)reduces behavioural indices of anxiety without significantly altering general activity measures, and further showed that this effect was completely blocked by intra-PAG pretreatment with an intrinsically-inactive dose of ketanserin (10 nmol/0.1 μl). Together, these results suggest that 5HT2 receptor populations located within the midbrain PAG play a significant role in the reduction of anxiety observed following disinhibition of 5-HT neurons in the MRN. | |
dc.publisher | Universidade Federal de São Carlos | |
dc.publisher | BR | |
dc.publisher | UFSCar | |
dc.publisher | Programa Interinstitucional de Pós-Graduação em Ciências Fisiológicas - PIPGCF | |
dc.rights | Acesso Aberto | |
dc.subject | Ansiedade | |
dc.subject | Labirinto em cruz elevado | |
dc.subject | 5-HT2 | |
dc.subject | Substância cinzenta periaquedutal | |
dc.subject | Camundongo | |
dc.title | Bloqueio dos receptores 5-HT2 da substância cinzenta periaquedutal suprime o efeito ansiolítico resultante do antagonismo dos receptores 5-HT1A do núcleo mediano da rafe em camundongos | |
dc.type | Tesis | |