Dissertação
Efeito do cinamaldeído sobre o metabolismo de nucleotídeos e nucleosídeo da adenina e em modelo experimental em artrite por adjuvante
Fecha
2014-12-20Registro en:
THORSTENBERG, Maria Luiza Prates. Effect of cinnamaldehyde on nucleotide and nucleosise of adenina metabolism in lymphocytes in arthritis by adjuvant. 2014. 96 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2014.
Autor
Thorstenberg, Maria Luiza Prates
Institución
Resumen
Rheumatoid arthritis (RA) is a chronic, multisystem inflammatory disease with
autoimmune features evident, expressing a cellular response of Th1. Characterized
primarily by chronic synovitis, erosive and symmetrical, preferably peripheral joints,
where there is an intense inflammatory process. The purinergic signaling system plays
an important role in the modulation of inflammatory and immune responses through
extracellular biomolecules such as nucleotides adenine and adenosine derivative
nucleodise therefore, which are essential to providing the trigger and maintain the trigger
inflammatory response. The effects of these molecules are promoted by the action of
purinergic receptors specific and controlled by ectoenzymes, in cell surface. Based on
these principles, this study investigated the effect of cinnamaldehyde in thermal
hyperalgesia, , arthritis score, paw edema and thermal hyperalgesia as well histolgical
parameters beyond the activity of the E-NTPDase and E-ADA in lymphocytes in rats
adjuvant arthritis. The rats were divided in four groups, of wich two were adjuvant
induced arthritis in the other two control groups. The animal received the compound at a
concentration of 2,1% orally for a period of 15 days. Not differences were observed
among analysis the arthritis score, paw edema it is however noted differences in thermal
hyperalgesia in about 60% and in group induced arthritic and treated with
cinnamaldehyde. Compared histological analysis it was noticed a slight reduction the
lynphocitytic inflammatory infiltration in rats induced arthritis and treated with
cinnamaldehyde. We found the increased the ATP hydrolysis in about 94,14% in arthritis
induced when compared with control group and 20,58% when compared with groups
treated with cinnmaldehyde . However, E-NTPDase activity when used ADP as substrate
rised in 152,56% in relation the control group and 122,76% in relation the control group
treated with cinnamaldehyde. In E-ADA activity was observed the increased in about
151,84%%in group arthritis induced when compared with control groups and rise 69,7%
when compared with group arthritis induced treated with cinnamakdehyde. In conclusion,
the data indicate that cinnamaldehyde was able to reduce thermal hyperalgesia and
alterations histological in rats induced arthritis, as well as decreased the
ectonucleotidase cascade, once we observed a activity gradual decrease in lymphocytes
in group induced arthritis and treated with cynnamaldehyde in relation with all others
groups. Therefore cinnamaldehyde was act to skewing some effects inflammatory of
induced arthritis. Although requiring further study, cinnamaldehyde could be used as a
complementary fot the benefit of people with rheumatoid arthritis.