Dissertação
Isolamento da β-lactoglobulina do soro do leite por cromatografia iônica
Fecha
2013-06-28Registro en:
PELEGRINI, Susana Berleze de. Isolation of β-lactoglobulin from whey by ion chromatography. 2013. 57 f. Dissertação (Mestrado em Ciência e Tecnologia dos Alimentos) - Universidade Federal de Santa Maria, Santa Maria, 2013.
Autor
Pelegrini, Susana Berleze de
Institución
Resumen
The allergy to the cow s milk protein affects 2 to 5% from the infantsand its symptoms
can vary from minor to life threatening. β-lactoglobulin is the protein fraction more
allergenic from the whey. Many processes were developed for the separation and
purification of proteins and among them is the ion exchange chromatography. The
objective of this work was to obtain the separation of β-lactoglobulin from the whey
protein concentrate by ion exchange chromatography, aiming the application in milk
formulas. Physical-chemical analysis of the protein concentrate, separation of β-
lactoglobulin by ion exchange chromatography, analysis of amino acids profile and of
the final amount of β-lactoglobulin in the resulting product and in two hypoallergenic
infant formulas were performed. From the results in this study, it is possible to
ascertain that it was feasible to separate the protein fractions of β-lactoglobulin by the
ion chromatography process.The protein obtained by this process was proven to be a
great amino acid source compared to the hypoallergenic infant formulas.