Dissertação
Verbena litoralis Kunth (Verbenaceae) micropropagação, estaquia e análise de flavonóides e cumarinas
Fecha
2013-07-31Registro en:
BERTÊ, Rosiana. Verbena litoralis Kunth (Verbenaceae) micropropagation, cutting and analysis and flavonoids and cumarins. 2013. 79 f. Dissertação (Mestrado em Ciências Biológicas) - Universidade Federal de Santa Maria, Santa Maria, 2013.
Autor
Bertê, Rosiana
Institución
Resumen
The species Verbena litoralis Kunth is popularly known as wort or father-caetano
gervãozinho field, and southern Brazil is used as detoxifying, antipyretic and diarrhea. The
aim of this study was to vegetative propagation through cuttings and micropropagation of
V. litoralis, evaluate the physiological parameter settings as chlorophyll fluorescence,
photosynthetic pigments and identify the phytochemical compounds present in the extracts
of plants obtained in vitro after acclimatization of the same. The experiment was conducted
using micropropagation complete MS plus 0.1 ml L-1 fungicide (carbendazim®), 30 g L-1
sucrose, 6.0 g L-1 agar and four combinations of 6-benzylaminopurine (BAP) and
naphthaleneacetic acid (NAA): 0, 1.0, 2.0, 4.0 and 0, 0.1, 0.2, 0.4 mg L-1, respectively, and
presence or absence of coal enabled (1 mg L-1). The plants obtained in vitro were
transferred to disposable cups and kept in a growth chamber throughout the experiment
with a temperature of 25 ± 2 º C and 16 hours photoperiod. The phytochemical analysis of
the extract of the aerial parts and roots of V. litoralis, obtained by maceration hydroethanol
was performed by HPLC and detected percentages of near 4.76% rutin, quercetin 9.18%
and 0.031% coumarin. In micropropagation, the absence of BAP and NAA led to a
percentage of 21% of grown plants. The acclimatization showed homogeneous
development since all were subjected to the same substrate type and temperature.