Tesis
Controle genético da coloração do tegumento e da concentração de ferro em sementes de feijão mesoamericano e andino
Fecha
2014-02-24Registro en:
POSSOBOM, Micheli Thaise Della Flora. Genetic control of seed coat colorand of iron concentration in middle american and andean common bean seeds. 2014. 73 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Santa Maria, Santa Maria, 2014.
Autor
Possobom, Micheli Thaise Della Flora
Institución
Resumen
The acceptance of the beans by consumers is related to seed coat color and nutritional
value. The objectives of this work were: (1) to verify the existence of maternal effect for the
color parameters "L", a* and b* and for the iron concentration in Middle American and
Andean common bean seeds; (2) to investigate the distribution of iron in the seed coat and
embryo of the Middle American and Andean common bean seeds; (3) to estimate heritability
and gain with the selection for the parameters "L", a* and b* and for the iron concentration in
common bean seeds of different gene pools; and (4) to select recombinant with seed coat
color of highest commercial demand and with high iron concentration in the seeds. To do this,
were performed controlled crosses between Middle American lines CNFP 10104 and CHC
01-175, and Andean lines Cal 96 and Hooter to obtain F1, F1 reciprocal, F2, F2 reciprocal and
backcrosses (RC11 e RC12) generations for each hybrid combination. Parents and generations
obtained were evaluated in two field experiments (normal rainy and dry season) in Santa
Maria - RS. Significant maternal effect was obtained for all color parameters in the Middle
American and Andean gene pool. In CNFP 10104 x CHC 01-175 crossing high broad sense
heritability estimates were obtained for luminosity (h2b: 76.66 to 78.09%), chromaticity a*
(h2b: 73.08 to 89.31%) and chromaticity b* (h2b: 88.63 to 90.11%) values in normal rainy and
dry season. In Cal 96 x Hooter crossing the parameters luminosity (h2b: 95.07%),
chromaticity a* (h2b: 86.19%) and chromaticity b* (h2b: 92.50%) showed high broad sense in
dry season. For iron concentration, maternal effect was significant only for the Middle
American gene pool. The iron concentration was higher in seed coat of Middle American
lines, and superior in embryo of Andean lines. Estimates of narrow sense heritability for the
iron concentration of 54.69% and broad sense heritability of 70.20% for CNFP 10104 x CHC
01-175 crossing and broad sense heritability of 62.27% for Cal 96 x Hooter crossing were
observed. A F2 plant with iron concentration of 132.99 mg kg- 1 of dry matter was obtained
from CNFP 10104 x CHC 01-175 crossing. The selection of F3 seeds (seed coat in F2) from
the crossings performed should be promising to obtain common bean lines with seeds colored
according to the market demand and with high iron concentration in seeds.