Dissertação
Atividade das enzimas ntpdase, 5´-nucleotidase e adenosina deaminase em plaquetas de ratos infectados por Trypanosoma evansi
Fecha
2010-08-12Registro en:
OLIVEIRA, Camila Belmonte. Activity of the enzymes ntpdase, 5´-nucleotidase and adenosine deaminase in platelets of rats infected with Trypanosoma evansi. 2010. 68 f. Dissertação (Mestrado em Medicina Veterinária) - Universidade Federal de Santa Maria, Santa Maria, 2010.
Autor
Oliveira, Camila Belmonte
Institución
Resumen
Nucleotide- and nucleoside-degrading enzymes are present in the surface of platelets,
blood cells involved in clotting disturbances of Trypanosoma evansi-infected animals.
Thus, this study was aimed at evaluating the activity of the enzymes NTPDase, 5 -
nucleotidase and adenosine deaminase in platelets of rats experimentally infected by T.
evansi. Animals were divided into four groups, according to the degree of parasitemia.
Samples were collected at days 3 (group A: at the beginning of parasitemia), 5 (group B:
high parasitemia) and 15 (group C: chronic infection). Group D (control group) was
composed of non-infected animals. Blood samples with citrate as the anticoagulant were
collected and used for platelet separation and enzymatic assays. NTPDase, 5 -
nucleotidase and adenosine deaminase (ADA) activities were decreased (p<0.05) in
platelets from rats of groups A and B, when compared to the control group. In group C,
only NTPDase and 5 -nucleoside activities were decreased (p<0.001), observed by ADP
and AMP hydrolysis. The correlation between platelet count and nucleotide and
nucleoside hydrolysis was positive and statistically significant (p<0.05) in groups A and
B. Platelet aggregation of all infected groups was decreased in comparison to the
control group (p<0.05). Based upon the results, it is concluded that the alterations
observed in the activity of the enzymes NTPDase, 5 -nucleotidase and adenosine
deaminase in platelets of T. evansi-infected animals might be related to
thrombocytopenia.