Isolamento e cultura de células tronco mesenquimais da polpa dental e comparação de implantes dentários para cães estudos - estudos in vitro

Abstract

This work was developed in two stages, described in scientific papers format. The goal of the first article was to adjust the techniques described in the literature for the isolation and culture of dental pulp cells to the physical and economic conditions of the research laboratory where the research was conducted. The methodology of this study was oriented by obtaining the pulp tissue from five deciduous teeth of dogs, which were digested in simple solution collagenase type 1 with positive results for the isolation. The outcome of the study was the isolation and cell culture of dental pulp with a lower cost and with enough cells for use in therapy. In the second study, the objective was to compare the in vitro resistance of dental implants with masticatory force dogs reported in the scientific literature. For materials and methods three different diameters dental implants 3.3mm, 4mm and 5mm were tested for static strength by applying compressive force on them. The result was a direct relationship to the resistance between the diameter and the applied force. Therefore, we can conclude that the method of isolation of dental pulp cells and culture of this study is similar to that described in the literature, but with the simplified methodology. And chewing strength of dogs can vary greatly, therefore we recommend possible the use of dental implants with a larger diameter.