Dissertação
Análise de estimulantes em suplementos alimentares e produtos naturais a base de plantas comercializados para fins de emagrecimento no Brasil
Fecha
2015-02-27Registro en:
ZEMOLIN, Gabriela Mezzomo. Analysis of stimulants in dietary supplements and
plant-based natural products marketed for weight
loss in Brazil. 2015. 115 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal de Santa Maria, Santa Maria, 2015.
Autor
Zemolin, Gabriela Mezzomo
Institución
Resumen
The strong advertising appeal, the search for a good physique, and the improvement in
physical performance, has risen the popularity of food supplements and other therapies with
slimming supplements based on plant extracts. Since the law exempts food supplements
from registration, they are easy targets for importation, commercialization e consumption by
the population, which worsens malpractice cases of adulterations. Thus, the objectives of the
present work were: research the presence of stimulants in food supplements for both
slimming and weight gain; study the addition of synthetic caffeine in natural products based
on plants used for weight loss; develop chromatographic methods using HPLC with
photodiode detection to detect caffeine, salicin, ephedrine, hordenine, tyramine, octopamine
and synephrine (including the L and D enantiomers). The stimulants were separated by
reverse phase chromatography using octadecylsilane columns (C18). For L and D
synephrine, chromatography with chiral phase of β-cyclodextrin was used. Detection limits
varied from 0.02 mg L-1 to 0.35 mg L-1, while quantification limits ranged from 0.07 mg L-1 to
1.16 mg L-1 for the separation of stimulants in food supplements. For analyses of caffeine in
herbal products, the LOD and LOQ were 0.048 mg L-1 and 0.16 mg L-1, respectively. For the
anlysis of the enantiomers of p-synephrine, the L enantiomer presented a detection limit of
0.11 mg L-1 and quantification limit of 0.36 mg L-1, while the D enantiomer exhibited a
detection limit of 0.41 mg L-1 and a quantification limit of 1.36 mg L-1. Each method
developed was then applied to the sample, which were, dietary supplement samples (n =
47), herbal products (n = 100), and fruits of Citrus aurantium. Caffeine was the most frequent
of the studied stimulants, often exceeding the labeled content and the maximum dose per
day recommended (420 mg). The food supplements presented caffeine, synephrine and
ephedrine as main stimulants in 53% of the studied samples.