Artículos de revistas
Resistance of melanized yeast cells of Paracoccidioides brasiliensis to antimicrobial oxidants and inhibition of phagocytosis using carbohydrates and monoclonal antibody to CD18
Fecha
2009-07-01Registro en:
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 104, n. 4, p. 644-648, 2009.
0074-0276
S0074-02762009000400019.pdf
S0074-02762009000400019
10.1590/S0074-02762009000400019
WOS:000269466900019
Autor
Silva, Marcelo Barbosa da
Thomaz, Luciana
Marques, Alexandre Ferreira
Svidzinski, Artur Estivalet
Nosanchuk, Joshua Daniel
Casadevall, Arturo
Travassos, Luiz Rodolpho
Taborda, Carlos Pelleschi
Institución
Resumen
Paracoccidioides brasiliensis, a thermal dimorphic fungal pathogen, produces a melanin-like pigment in vitro and in vivo. We investigated the involvement of carbohydrates and monoclonal antibody to CD18, on phagocytosis inhibition, involving macrophage receptors and the resistance of melanized fungal cells to chemically generated nitric oxide (NO), reactive oxygen species (ROS), hypochlorite and H2O2. Our results demonstrate that melanized yeast cells were more resistant than nonmelanized yeast cells to chemically generated NO, ROS, hypochlorite and H2O2, in vitro. Phagocytosis of melanized yeast cells was virtually abolished when mannan, N-acetyl glucosamine and anti-CD18 antibody were added together in this system. Intratracheal infection of BALB/c mice, with melanized yeast cells, resulted in higher lung colony forming units, when compared to nonmelanized yeast cells. Therefore, melanin is a virulence factor of P. brasiliensis.