Two new complete genome sequences contribute to the biology of Lysinibacillus sphaericus

Abstract

Lysinibacillus sphaericus OT4b_25 and are both native Colombian strains the first one isolated from coleopteran larvae and the second one from soil samples Both strains have shown high evels of pathogenic activity against Culex quinquefaciatus larvae in laboratory assays compared to other members of the same species Using Pacific Biosciences sequencing technology we sequenced annotated (de novo) and described their genomes achieving a complete genome sequence status on both cases We then performed a comparative analysis between the newly sequenced genomes and the ones previously reported for Colombian isolates L. sphaericus OT4b_31 and CBAM5 with the inclusion of L sphaericus C3-41 that has been used as a reference genome for most of previous genome sequencing projects We concluded that L. sphaencus OT4b_25 and are highly similar amongst themselves and share high levels of synteny with strains CBAM5 and C3-41