Artículos de revistas
The expression of heterologous MAM-7 in Lactobacillus rhamnosus reduces its intrinsic capacity to inhibit colonization of pathogen Vibrio parahaemolyticus in vitro.
Fecha
2016Registro en:
ISSN 0716-9760
10.1186/s40659-015-0064-1
Autor
Munoz-Bergmann,Cristian A [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]
Elola-López, Ana [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]
Quintana, Javiera [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]
Segovia,Cristopher [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]
Trombert, Annette N. [Chile. Universidad Mayor. Centro de Genómica y Bioinformática]
Institución
Resumen
BACKGROUND: Vibrio parahaemolyticus (V. parahaemolyticus) is a Gram-negative, halophilic bacterium recognized as one of the most important foodborne pathogen. When ingested, V. parahaemolyticus causes a self-limiting illness (Vibriosis), characterized mainly by watery diarrhoea. Treatment is usually oral rehydration and/or antibiotics in complicated cases. Since 1996, the pathogenic and pandemic V. parahaemolyticus O3:K6 serotype has spread worldwide, increasing the reported number of vibriosis cases. Thus, the design of new strategies for pathogen control and illness prevention is necessary. Lactobacillus sp. grouped Gram positive innocuous bacteria, part of normal intestinal microbiota and usually used as oral vaccines for several diarrheic diseases. Recombinants strains of Lactobacillus (RL) expressing pathogen antigens can be used as part of an anti-adhesion strategy where RL block the pathogen union sites in host cells. Thus, we aimed to express MAM-7 V. parahaemolyticus adhesion protein in Lactobacillus sp. to generate an RL that prevents pathogen colonization. RESULTS: We cloned the MAM-7 gene from V. parahaemolyticus RIMD 2210633 in Lactobacillus expression vectors. Recombinant strains (Lactobacillus rhamnosus pSEC- AM7 and L. rhamnosus pCWA-MAM7) adhered to CaCo-2 cells and competed with the pathogen. However, the L. rhamnosus wild type strain showed the best capacity to inhibit pathogen colonization in vitro. In addition, LDH-assay showed that recombinant strains were cytotoxic compared with the wild type isogenic strain. CONCLUSIONS: MAM-7 expression in lactobacilli reduces the intrinsic inhibitory capacity of L. rhamnosus against V. parahaemolyticus
Materias
Ítems relacionados
Mostrando ítems relacionados por Título, autor o materia.
-
Empathy in students of specialty in physical therapy, universidad mayor in temuco, Chile [Empatía em estudiantes de kinesiología. Universidad Mayor, sede Temuco, Chile] [Empatía en estudiantes de kinesiología. Universidad Mayor, sede Temuco, Chile]
Díaz-Narváez V.P.; Salas-Alarcón D.; Bracho-Milic F.; Ocaranza-Ozímica J. (Universidad del Rosario, 2015) -
Empathy in nursing students at the universidad mayor, Temuco campus, Ix region, Chile [Empatia em estudantes de enfermagem da universidad mayor, Sede Temuco, Ix região, Chile] [Empatía en estudiantes de enfermería de la universidad mayor, Sede Temuco, Ix región, Chile]
Díaz-Narváez V.P.; Muñoz-Gámbaro G.; Duarte-Gómez N.; Reyes-Martínez M.C.; Caro S.E.; Calzadilla-Núñez A.; Alonso-Palacio L.M. (Universidad de La Sabana, 2014) -
Estudio comparativo de algunas características demográficas y epidemiológicas, de los pacientes adultos mayores desdentados totales atendidos en la clínica de prótesis totales de la Facultad de Odontología Universidad de Chile y en el Centro de Referencia Maruri durante el año 2006
Yáñez Núñez, Ruth Isabel (Universidad de Chile, 2007)Objetivo: Analizar si existen diferencias en algunas de las características Demográficas y Epidemiológicas estudiadas de los pacientes adultos mayores desdentados totales atendidos en la clínica de Prótesis Totales de ...