Artículos de revistas
The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development
Fecha
2016-07Registro en:
Nature Communications 7, Article number:12119 (2016)
2041-1723
doi:10.1038/ncomms12119
Autor
Rautengarten, Carsten
Ebert, Berit
Liu, Lifeng
Stonebloom, Solomon
Smith-Moritz, Andreia M.
Pauly, Markus
Orellana, Ariel
Scheller, Henrik Vibe
Heazlewood, Joshua L.
Institución
Resumen
Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.