Artículos de revistas
Técnicas de Neurorrafia: Estudio Experimental Estereológico Axonal Comparativo
Fecha
2015-01-01Registro en:
International Journal of Morphology, v. 33, n. 3, p. 1002-1008, 2015.
0717-9502
0717-9367
10.4067/S0717-95022015000300031
2-s2.0-84943389510
Autor
University Camilo Castelo Branco, UCCB and Foundation Education of Fernandópolis, FEF - Fernandópolis
São José do Rio Preto Medical School, FAMERP
Universidade Estadual Paulista (Unesp)
Camilo Castelo Branco University
University Camilo Castelo Branco and Education Foundation of Fernandópolis
Camilo Castelo Branco University, and Education Foundation of Fernandópolis
Anhembi Morumbi University
Institución
Resumen
There is a great variety of injuries that affect peripheral nerves derived from acquired or congenital degenerative diseases affecting the central nervous system that cause loss of sensorimotor functions. The objective of this work was to perform an endto- side or side-to-side experimental axonal stereological study in order to compare volume density of axons, endouneuro and myelin sheath (and muscle mass) in peroneal and tibial nerves, with anastomosis contact from 0.25 cm to 0.50 cm. After approval of the Ethics Committe, 20 male Wistar rats were divided into four groups of five rats each (G1= end-to-side neurorrhaphy; G2= side-to-side neurorrhaphy of 0.25 cm; G3= side-to-side neurorrhaphy of 0 cm and G4= Control of normality). After 180 days, fragments of peroneal and tibial nerves were collected for histological and stereological study. In comparative stereological experimental study between neurorraphies, the volume density of axons, myelin sheath of tibial and fibular nerves, as well as the post-surgical muscle mass, remains the same in end-to-side and side-to-side neurorraphies, regardless of contact area of anastomosis. It can be inferred, as surgical repair options, both end-to-side neurorrhaphy to recover and prevents atrophy of the endplate as side-to-side neurorraphy that is independent of the distance between the nerve stumps.