Artículos de revistas
Galectin-3: Role in ocular allergy and potential as a predictive biomarker
Fecha
2018-07-01Registro en:
British Journal of Ophthalmology, v. 102, n. 7, p. 1003-1010, 2018.
1468-2079
0007-1161
10.1136/bjophthalmol-2017-311473
2-s2.0-85049136750
2-s2.0-85049136750.pdf
Autor
Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Institución
Resumen
Aims To evaluate galectin-3 (Gal-3), a β-galactoside binding protein, as a possible biomarker in ocular allergy and further investigated the role of endogenous Gal-3 in a murine model of ovalbumin (OVA)-induced allergic conjunctivitis (AC). Methods Conjunctival impression cytology specimens from control and patients with severe vernal keratoconjunctivitis, treated or untreated, were used to evaluate Gal-3 expression by immunocytochemistry. To investigate the mechanism of action of Gal-3, OVA-immunised BALB/c male wild-type (WT) and Gal-3 null (Gal-3 -/-) mice were challenged with eye drops containing OVA on days 14-16 with a subset of animals pretreated with 0.03% tacrolimus (TC) or dexamethasone (Dex). Results Patients with AC and OVA-sensitised WT mice exhibited increased levels of Gal-3 in the conjunctiva compared with control, an effect reverted by the action of Dex and TC therapy. Twenty-four hours after the final OVA challenge, total and anti-OVA IgE levels increased significantly in the blood of OVA-sensitised WT and Gal-3 -/- mice compared with controls, supporting the efficacy of the AC model. The lack of endogenous Gal-3 exacerbated the local inflammatory response, increasing the influx of eosinophils and mast cell activation. Additionally, OVA-sensitised Gal-3 -/- animals exhibited increased CD4 + expression in the eyes as well as eotaxin, IL-4, IL-13 and interferon-3 levels in the tear fluid compared with WT animals. Conclusion Gal-3 contributes to the pathogenesis of ocular allergy and represents a relevant therapeutic target.